“Proliferation Monitoring by Flow Cytometry”

The second module in Lesson 2 of the course series

Lesson 2:   Monitoring Cell Cycle Progression

Module B:  DNA Cell Cycle Data Analysis Strategies

This is the second of four modules of Lesson 2 of the Proliferation course.  Module 2B provides an in depth discussion of DNA cell cycle data analysis strategies. The underlying concepts behind the characteristic cell cycle distribution are described followed by a description with examples of simple and complex approaches to modeling normal and abnormal DNA fluorescence data.

The course concludes with a discussion of controls to include in proper experimental design and explains how to analyzed synchronized cell populations as seen when cell cultures are drug treated.

Lesson 1: Basics of the Cell Cycle is currently available here.

Leasson 2A: Principles and Methods is currently available here.


Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.

Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA

Paul K. Wallace - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.

Target Audience

Individuals with a basic understanding of flow cytometry who are unfamiliar with, or wish to review, how DNA flow cytometric data is analyzed.

CYTO University

CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.


Seminar Information
Seminar Date:
January 31, 2018
Proliferation Monitoring by Flow Cytometry - Lesson 2 - Module B - Data Analysis Strategies
ISAC Member Price: $0.00
Non-Member Price:$25.00
Individual topic purchase: Selected
American Society for Clinical Pathology
CMLE: 1.00
ISAC Member Price: $0.00
Non-Member Price:$25.00