CYTO U Upcoming Webinars
Collapse Fluorescent Retroviruses as Reference Particles for Nanoscale Flow Cytometry

Fluorescent Retroviruses as Reference Particles for Nanoscale Flow Cytometry

Tuesday, February 26, 2019 at 12 pm EST (US & Canada)

Presented by

 Vera A. Tang, Ph.D.

Operations Manager & Adjunct Professor

University of Ottawa Flow Cytometry & Virometry Core Facility

Moderated by

Joshua A. Welsh, Ph.D.

Visiting Postdoctoral Fellow

Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute

 

About the Faculty

Vera helped to establish the Flow Cytometry & Virometry Core Facility at the University of Ottawa in 2013 when she became the first operations manager of the facility. Through her role in the core facility, she has channeled her passion for acquiring and sharing knowledge into teaching and research. Aside from new user training, she has developed courses in flow cytometry for undergraduate and graduate programs at the Faculty of Medicine at the University of Ottawa. Her research involves the development of retroviruses for use as fluorescence standards, as well as developing protocols for analysis of viruses and EVs by small particle flow cytometry. This work has led to the formation of a company of which Vera is a founding member and CSO. Vera is an active member of the cytometry community. She was accepted into the ISAC Shared Resource Lab Emerging Leaders Program in 2018 and is also currently serving as the co-president of the Canadian Cytometry and Microscopy Association.

Webinar Summary

This webinar will Introduce fluorescent retroviruses as potential fluorescence reference particles and how they can be used in small particle flow cytometry.  It will also discuss features of viruses that are unique from currently available small particle reference materials and showcase new projects currently underway for development of MESF viruses.

Learning Objectives

-Improve understanding of the challenges for small particle flow cytometry

-Emphasize the need for reference materials and reagent development in small particle flow cytometry

-Introduce flow cytometrists to flow virometry

Who Should Attend

Anyone who has an interest in the analysis of small particles such as EVs and viruses

Formats Available: Streaming, Live Webcast + Streaming
Original Seminar Date: February 26, 2019
On-Demand Release Date: February 26, 2019

Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Fluorescent Retroviruses as Reference Particles for Nanoscale Flow Cytometry
    Collapse The Art of Dimensionality Reduction: What Really Matters

    The Art of Dimensionality Reduction: What Really Matters

    Thursday, March 28th, 2019 at 12:00 pm EST (US & Canada)

    Presented by

    Karel-Drbal

     

    Karel Drbal, PhD

    Professor

    Department of Cell Biology, Faculty of Science, Charles University, Prague, Czechia

    About the Faculty

    Karel Drbal obtained a MSc in Cell Biology and PhD in Immunology from Charles University in 1991 and 2000, respectively. For most of his career he has been specialized in monoclonal antibody generation and characterization (MEM-series) by flow cytometry. After postdoctoral fellowships with Dr. Hannes Stockinger at Medical University Vienna and Dr. Vaclav Horejsi at Institute of Molecular Genetics of CAS, he held the CSO position (2010-2013) in biotech enterprise Exbio Praha. Since 2013 he is teacher of Immunology, Systems Biology and Cytometry, and in 2015 he launched the lab of Molecular dynamics of the immune response at Charles University. His studies in the field includes the following:

    • -Focus on clinical collaboration and precision medicine approach
    • -Analysis of microevolution of the immune system in response to certain human pathologies correlated with high mutation rates of tumor cells or pathogens
    • -Research and technological development of large-scale generation of binding proteins (monoclonal antibodies and engineered alternatives)
    • -Multivariate data analysis, image analysis and structural bioinformatics

     

    Webinar Summary

    I will start with brief introduction to the state-of-the-art dimensionality reduction algorithms (SNE variants and UMAP) for unsupervised cytometry data analysis. Next, these algorithms will be compared side by side to the new EmbedSOM algorithm based on FlowSOM clustering approach. Shortly, the importance of input data quality, the logic of clustering and embedding workflow and the output annotation will be described with the examples in R environment. Finally, the results of benchmarking datasets as well as few use cases analyzed by hierarchical dissection of the embedded data will be presented. To close, the comparison to manual workflow and the subjective visual perception of the output quality will be discussed, and the future directions of unsupervised vs. supervised analysis beyond the field of cytometry will be outlined.

    Learning Objectives:

    • -Discuss the principle of the new EmbedSOM algorithm and the associated workflow
    • -Learn the differences between the embedding algorithms for data visualization
    • -Describe hierarchical dissection of complex datasets including statistical output
    • -Discuss the strengths and weaknesses of the unsupervised process

    Who Should Attend

    • -Clinical and research scientists collecting multidimensional data not only in the field of cytometry, but also microscopy, transcriptomics, and proteomics
    • -Computational scientists and bioinformatics core personnel interested in unsupervised data visualization

     

    Formats Available: Streaming, Live Webcast + Streaming
    Original Seminar Date: March 28, 2019
    On-Demand Release Date: March 28, 2019

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information The Art of Dimensionality Reduction: What Really Matters
    Collapse Platelet Flow Cytometry

    Platelet Flow Cytometry

    Wednesday, July 31st, 2019 at 9 am EST (US & Canada)

    Presented by

    Matthew Linden, PhD

    Associate Professor

    University of Western Australia

     

    About the Faculty

    Matthew is the Associate Professor of Haematology at The University of Western Australia, where is leads the development and delivery of haematology and cytometry education to train hospital scientists. Matthew’s research interest is in platelet biology and function. Working at the interface of the shared resource laboratory, discovery and translational research, Matthew has developed novel cytometry techniques for the measurement of blood platelets and employed these in the development of new antiplatelet therapies. He is committed to advancing cytometry through strong, sustainable shared resource laboratories and cytometry education. Matthew is an ISAC Marylou Ingram Scholar and the current President of the Australasian Cytometry Society.

    Webinar Summary

    Flow cytometry is a powerful and versatile tool which can be used to provide substantial phenotypic data on platelets including surface expression of functional receptors, bound ligands, expression of granule components, signal transduction, platelet-platelet aggregation or interaction of platelets with leukocytes. Quantitative assessment of these parameters may facilitate the diagnosis of inherited or acquired platelet disorders, assist in the diagnosis of diseases associated with platelet activation, or assist in the monitoring of safety and efficacy of antiplatelet therapy.

    Learning Objectives:

    -Discuss the utility of platelet flow cytometry

    -Describe the process by which platelet phenotype and function are measured

    -Discuss the pre-analytical and analytical variables that can affect platelet data

    Who Should Attend

    Clinical and research scientists who wish to measure platelet phenotype and function.

     

    Formats Available: Streaming, Live Webcast + Streaming
    Original Seminar Date: July 31, 2019
    On-Demand Release Date: July 31, 2019

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Platelet Flow Cytometry
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