CYTO U Courses
Collapse FINAL Authors Review Demo:  Apoptosis - Module 1:  Background and Basic Techniques
Formats Available: Author Demo
Original Seminar Date: September 24, 2018
On-Demand Release Date: Available Now
Topics & Pricing InformationTopics & Pricing Information FINAL Authors Review Demo:  Apoptosis - Module 1:  Background and Basic Techniques
Collapse Proliferation Monitoring, Lesson 2D:  Applications

Proliferation Monitoring by Flow Cytometry

The fourth and final module in Lesson 2 of the course series.

Lesson 2: Monitoring Cell Cycle Progression

Module D: Applications

This is the final module in Lesson 2 of the Proliferation Course, which focuses on cell cycle analysis by flow cytometry. Module 2D “Applications" looks at how investigators have used the principles and methods discussed in Lessons 2A – 2C to monitor cell cycle status and cell cycle progression in their research fields of interest. Topics covered include tumor diagnosis and prognosis, anti-tumor mechanisms of therapeutic agents, and regulation of growth vs. quiescence or senescence in normal and neoplastic cells. 

Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

Lesson 2A: Principles and Methods is currently available here.

Lesson 1: Basics of the Cell Cycle is currently available here.

Authors

Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.

Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA

Paul K. Wallace - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.

CYTO University

CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.

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To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.

For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

 

This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

Formats Available: Proliferation Monitoring, Lesson 2D: Applications
Original Seminar Date: May 11, 2018
On-Demand Release Date: Available Now

Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 2D:  Applications
    Collapse Proliferation Monitoring, Lesson 2C:  Practical Considerations & Troubleshooting

    Proliferation Monitoring by Flow Cytometry

    The third module in Lesson 2 of the course series.

    Lesson 2: Monitoring Cell Cycle Progression

    Module C: Practical Considerations & Troubleshooting

    This is the third of four modules in Lesson 2 of the Proliferation Course, which focuses on cell cycle analysis by flow cytometry. Module 2C: “Practical Considerations and Troubleshooting" examines how choices made during sample preparation, reagent selection, instrument set-up, and data collection impact the reliability and quality of the resulting DNA histograms. Topics covered include instrument linearity, fixation effects, doublet discrimination techniques, and special considerations for DNA cell cycle analysis using vital DNA dyes and thymidine analogs.

    Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

    Lesson 2A: Principles and Methods is currently available here.

    Lesson 1: Basics of the Cell Cycle is currently available here.

    Authors

    Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.

    Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA

    Paul K. Wallace - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.

    CYTO University

    CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.

    -------

    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

    If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.

    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

     

    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

    Formats Available: Proliferation Monitoring, Lesson 2C: Practical Considerations & Troubleshooting
    Original Seminar Date: March 17, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 2C:  Practical Considerations & Troubleshooting
    Collapse Proliferation Monitoring, Lesson 2B: Data Analysis Strategies

    Proliferation Monitoring by Flow Cytometry

    The second module in Lesson 2 of the course series

    Lesson 2: Monitoring Cell Cycle Progression

    Module B: DNA Cell Cycle Data Analysis Strategies

    This is the second of four modules of Lesson 2 of the Proliferation course.  Module 2B provides an in depth discussion of DNA cell cycle data analysis strategies. The underlying concepts behind the characteristic cell cycle distribution are described followed by a description with examples of simple and complex approaches to modeling normal and abnormal DNA fluorescence data.

    The course concludes with a discussion of controls to include in proper experimental design and explains how to analyzed synchronized cell populations as seen when cell cultures are drug treated.

    Lesson 1: Basics of the Cell Cycle is currently available here.

    Lesson 2A: Principles and Methods is currently available here.

    Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

     

    Authors

    Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.

    Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA

    Paul K. Wallace - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.

    Target Audience

    Individuals with a basic understanding of flow cytometry who are unfamiliar with, or wish to review, how DNA flow cytometric data is analyzed.

    CYTO University

    CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.

    -------

    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

    If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.

    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

     

    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

    Formats Available: Streaming
    Original Seminar Date: January 31, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 2B: Data Analysis Strategies
    Collapse Proliferation Monitoring, Lesson 2A: Principles and Methods

    Proliferation Monitoring by Flow Cytometry

    The first module in Lesson 2 of the course series

    Lesson 2: Monitoring Cell Cycle Progression

    Module A: Principles and Methods

    This is the first of four modules of Lesson 2 of the Proliferation course.  Module 2A focuses on the principles and methods for the use of DNA binding dyes alone, or in combination with thymidine analogs, proliferation related antigens, and/or phase specific expression vectors, to assess: 

    1. How cells within a population of interest are distributed among the different cell cycle phases; and
    2. The kinetics with which cells of interest are progressing through the cell cycle

    Lesson 1: Basics of the Cell Cycle is currently available here.

    Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

    Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

    Authors

    Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.

    Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA

    Paul K. Wallace - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.

    Target Audience

    Individuals with a basic understanding of flow cytometry who are unfamiliar with, or wish to review, how flow cytometry is used to monitor the distribution or progression of cells through the cell cycle.

    CYTO University

    CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.

     

    Formats Available: Streaming
    Original Seminar Date: August 30, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 2A: Principles and Methods
    Collapse Calibration, Lesson 1:  Overview and Checklist for Benchmarking
    Image Cytometer Characterization and Calibration - Lesson 1 of 3

     

    Welcome to the Image Cytometer Characterization and Calibration course.  This course contains three lessons.  The first lesson is "Overview and Checklist for Benchmarking". 

    This course has been developed for core facility managers, clinical personnel involved in quality assurance and scientists interested in reproducibility of imaging measurements.  Students should have a basic understanding of optical microscopy and hands-on experience with a fluorescence microscope.

    Formats Available: Online Course
    Original Seminar Date: April 10, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Calibration, Lesson 1:  Overview and Checklist for Benchmarking
    Collapse Proliferation Monitoring, Lesson 1: Basics of the Cell Cycle

    Proliferation Monitoring by Flow Cytometry

    Lesson 1: Basics of the Cell Cycle

    Lesson 1 of the Proliferation course reviews the basic concepts of cell cycle biology, setting the stage for the more specific methods and applications discussed in Lessons 2 and 3. Topics discussed include:

    • Phases of the cell cycle and molecular events associated with each
    • Cell cycle checkpoints and regulators (cyclins and cyclin dependent kinases)
    • The relationship between the cell cycle phase distribution of a sample and DNA content histogram
    • The use of DNA content histograms in combination with metabolic labeling or cellular synchronization to determine kinetics of progression through the different cell cycle phases

     

    Lesson 2A: Principles and Methods is currently available here.

    Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

    Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

    Authors

    Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.

    Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA

    Paul K. Wallace - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.

    Learning Objectives

    • To understand what cellular processes need to occur in order for a cell to enter the cell cycle and complete division. 
    • To know and understand the difference between cell cycle progression and cell division 
    • To understand how flow cytometry is used to measure cell cycle parameters and division

    CYTO University

    CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.

    -------

    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

    If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.

    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

     

    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

     

    Formats Available: Streaming
    Original Seminar Date: September 02, 2015
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 1: Basics of the Cell Cycle
    Collapse Flow Cytometry Biosafety Course

    Authors: Kevin Holmes, Steve Perfetto, Hank Pletcher, Ingrid Schmid

    The Flow Cytometry Biosafety course will provide a summary of biosafety principles as they apply to flow cytometry and cell sorting and provide an overview of applicable standards and risk assessment. The topics covered will be:

    • Biosafety Principles and Procedures
    • Understanding Risk Assessment
    • Instrument-specific Standard Operating Procedures (SOPs)
    • Biosafety in Cell Sorting
    • Containment Testing

    At the completion of this course, students will have a clearer understanding of the principles and practices of biosafety as it pertains to flow cytometry, in particular cell sorting. Additionally, you will have a list of resources to aid in risk assessment and the development of Standard Operating Procedures in your own lab. 

    To view the course, Click "Register Now."

    If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.  
     
    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can re-set it on the ISAC Web site by clicking on the "Forgot Password?" link on the log-in page.
    Formats Available: Streaming

    Approved Credit:
  • ASCP: 1.50 hours CMLE

  • Topics & Pricing InformationTopics & Pricing Information Flow Cytometry Biosafety Course