Shared Resource Lab
Collapse Finance 101: Equipment Maintenance

"Equipment Maintenance"

Part of the Finance 101 Webinar Series

Tuesday, February 20th at 12pm Eastern Time (US/Canada)

Presented by:

Julie Auger

Joanne Lannigan

 

More information on this free webinar will be posted soon!

 

Formats Available: Streaming, Live Webcast + Streaming
Original Seminar Date: February 20, 2018

Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Finance 101: Equipment Maintenance
    Collapse Finance 101: Rate Setting

    "Rate Setting"

    Part of the Finance 101 Webinar Series

    Monday, January 15th at 12pm Eastern Time (US/Canada)

    Presented by:

    Nicole White

    Peter O'Toole

     

    More information on this free webinar will be posted soon!

     

    Formats Available: Streaming, Live Webcast + Streaming
    Original Seminar Date: January 15, 2018

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Finance 101: Rate Setting
    Collapse Finance 101: Budgeting

    "Budgeting"

    Part of the Finance 101 Webinar Series

    Wednesday, December 13th at 12pm Eastern Time (US/Canada)

    Presented by:

    Julie Auger

    Nicole White

     

    More information on this free webinar will be posted soon!

     

    Formats Available: Streaming, Live Webcast + Streaming
    Original Seminar Date: December 13, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Finance 101: Budgeting
    Collapse SRL Best Practices Series, Part 7:  Staffing

    "Staffing"

    This is the seventh of seven webinars in our SRL Best Practices Series

    Presented by:


    Matthew Cochran        
    Technical Director, URMC Flow Cytometry SRL
    University of Rochester Medical Center
    Rochester, NY USA

     


    Robert Salomon
    Flow Cytometry Manager/Sr. Flow Cytometry  Scientist
    Garvan Institute of Medical Research
    Sydney, Australia

    About the Presenters:

    Matthew Cochran

    Matt is the Technical Director of the URMC Flow Cytometry SRL.  The lab contains eight traditional analytical flow cytometers, two cell sorters, an Image Stream X imaging cytometer and a CyTOF mass cytometer, serving the upstate NY region from Buffalo to Ithaca.  Matt began his career in flow cytometry in 2004 in the Rochester Human Immunology Core lab managing an 8-color LSRII and has been dedicated to the field ever since. He has experience with many traditional and non-traditional cytometers and a wide variety of applications including large-scale immunophenotyping and panel design.  In addition to the Rochester cytometry training program, which he’s been running for around eight years, he’s been teaching and consulting nationally for a number of years.  Matt is also a member of the ISAC shared resource laboratory task force, the ABRF flow cytometry research group, and the GLIIFCA steering committee.

    Robert Salomon

    Rob Salomon is the inaugural Technical Director of the Garvan-Weizmann Centre for Cellular Genomics. Rob has a background in biology and engineering and is interested in leveraging cutting edge technology to answer important biological questions. In 2014, he was named a Shared Resource Laboratory Emerging Leader by the International Society for the Advancement of Cytometry.  Since then, Mr. Salomon has played a major role in both the Australian and International Cytometry Societies.

    CYTO U Webinar Recordings

    A recording of this webinar will be posted online at CYTO U within 24-48 hours after the live event for free viewing by all. Recordings of previously presented live webinars are also available.
     

    CYTO University

    CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community.  In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics.  These are available at no cost to ISAC members and for a nominal charge for non-members.  Webinars are free for all.

     

    Formats Available: Recording Only
    Original Seminar Date: August 30, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information SRL Best Practices Series, Part 7:  Staffing
    Collapse SRL Best Practices Series, Part 6: Operations

    "Operations"

    This is the sixth of seven webinars in our SRL Best Practices Series

    Presented by:


    Joanne Lannigan        
    Director, Flow Cytometry Shared Resource
    University of Virginia
    Charlottesville, VA

     


    James Marvin
    Director, Flow Cytometry Core Facility
    University of Utah Health Sciences Center
    Salt Lake City, UT

    About the Presenters

    Joanne Lannigan, M.S.

    Joanne Lannigan has been actively involved in Flow Cytometry for over 30 years, where she has worked in clinical, industry and academic flow cytometry laboratories.  She is currently the Director of the Flow Cytometry Core Facility in the School of Medicine at the University of Virginia.  She has been an active member of ISAC since 1996 and served as an ISAC Council Member from 2012-2016, Chair of the Shared Resource Laboratory Task Force from 2014-2016, a member of the Shared Resource Laboratory Services Oversight Committee from 2014-2016 and Vice Chair of the Certification Advisory Committee from 2014-2016. She currently is the Chair of the SRL Content Task Force of the Education Committee as well as a member of the Cytometry Board of Certification Committee through ASCP.  Ms. Lannigan’s current interests involve advancing technologies in cytometry, small particle analysis by flow cytometry, instrument evaluation and quality assurance, flow cytometry education and core management.

    James Marvin

    James Marvin has spent virtually his entire career within a Flow Cytometry shared resource setting.  In 2001, James joined the bustling flow lab at the University of Chicago under the tutelage of Julie Auger and Ryan Duggan. This was the era in which cell sorters were a bit more “hands on” and a firm understanding of the underlying hardware was essential on a daily basis. Next, James moved on to Northwestern University to “pick the brain” of Dr. Charles Goolsby.  In addition to the routine aspects involved in a flow core, a transition to more clinical assays was pursued during this time. Through a strong collaboration with industry as well as academic partners, significant strides were made in optimizing assays related to signal transduction analysis in normal as well as diseased bone marrow. Finally, in 2011, James uprooted from the Midwest in search of the mountains and desert of Utah where he has been the Director of the Flow Lab at the University of Utah in Salt Lake City. One of the many unifying themes through each of these distinct labs was the emphasis that education and training played for both facility staff and clients.

    Webinar Summary

    One of the most important aspects of a well-run SRL is the operational structure of the facility. A properly managed SRL requires best practices that provide necessary oversight, strategic and fiscal management and planning, self-assessments, and external reviews for evaluating the facility’s performance. This webinar will discuss the infrastructure necessary to insure the efficient and effective operation of the SRL.

    Learning Objectives

    Upon completion of this webinar, you will have a greater understanding of the important operational components that need to be addressed as part of overall Best Practices of a SRL. These areas include: Management, Oversight, Performance Assessment, Annual Reports/Business Plans, Budget Development and Review, Facility Use Policy and Procedures, and communications.

     

    Formats Available: Recording Only
    Original Seminar Date: July 21, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information SRL Best Practices Series, Part 6: Operations
    Collapse SRL Best Practices Series, Part 5: Quality Control / Quality Assurance

    “Quality Control / Quality Assurance”

    This is the fifth of seven webinars in our SRL Best Practices Series

    Presented by:



    Lora Barsky
    Application Scientist, Flow Cytometry
    Beckman Coulter Life Sciences
    San Pedro, CA

     



    Rui Gardner, Ph.D.
    Head of Flow Cytometry Core Facility
    Memorial Sloan Kettering Cancer Center
    Greater New York Area

     

    Webinar Summary

    The importance of quality control and quality assurance within the SRL is critical to the overall function of such facilities.  Working in a “shared” environment in which a large variety of biological samples are introduced, requires a reasonable set of “best practices” to ensure the quality standards required of a successfully run shared facility.

    CYTO U Webinar Recordings

    A recording of this webinar will be posted online at CYTO U within 24-48 hours after the live event for free viewing by all.  Recordings of previously presented live webinars are also available.

    CYTO University

    CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, and interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.
     

    Formats Available: Streaming
    Original Seminar Date: June 28, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information SRL Best Practices Series, Part 5: Quality Control / Quality Assurance
    Collapse Implementing Best Practices in a Flow Cytometry Shared Resource (Core) Facility

    In November 2016, the ISAC Shared Resource Laboratory (SRL) Task Force published a document outlining a set of “best practices” for SRLs or Core Facilities to use as a general guide for achieving and maintaining standards of excellence in the services they provide. These best practices highlight several important areas that impact the efficiency, quality and reproducibility of services provided by these facilities. The purpose of this tutorial is to provide guidance and generate discussion and feedback on how to best implement these “best practices” across a wide variety and flavors of these SRLs or Cores.

    Outline:
    1.    Introduction of the Best Practices and what is hoped to be achieved through implementation
    2.    Brief presentation and background information on each topic including SOPs, Training and Education, Quality Assurance, Laboratory Safety, Data Management, Staffing, and Operations
    3.    Identification of potential roadblocks and hurdles and how to overcome them
    4.    Benefits of implementation and adherence to these best practices
     

    Formats Available: CYTO 2017 Scientific Session
    Original Seminar Date: June 10, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1.50 hours CMLE

  • Topics & Pricing InformationTopics & Pricing Information Implementing Best Practices in a Flow Cytometry Shared Resource (Core) Facility
    Collapse Establishing a New SRL Facility -- From Lab Design, Instrument Purchase, Understanding Your User Base

    Course Objective: 

    ?In establishing a new SRL Facility, or comprehensively upgrading an existing one, many factors have to be considered beyond the scope of purchasing an instrument and hiring someone to operate it. There is a wealth of expertise and published literature that can act as a guide, but major challenges arise in order to successfully implement the best practices needed to have a functional and productive SRL. It is the task of the new facility manager to gather detailed information about the need for the core facility, and refine this knowledge to get a realistic image and plan to set up and equip the shared resource lab.

    Outline Overview of Factors for Consideration:?

    • Selecting the right manager and support staff.

    • Ensuring the dedicated space fulfils the needs for now and the future, depending on the space allocated, or the space that can be made. ?

    • Understanding your user base, the direction of the Institute, the access to other SRLs, and basing instrument purchases and infrastructure construction around these.

    • Funding Considerations: Allocating and ensuring money streams to fulfil the needs and wants of the SRL.

    • Ancillary equipment and infrastructure considerations beyond flow cytometers.

    Formats Available: CYTO 2017 Scientific Tutorial
    Original Seminar Date: June 10, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1.50 hours CMLE

  • Topics & Pricing InformationTopics & Pricing Information Establishing a New SRL Facility -- From Lab Design, Instrument Purchase, Understanding Your User Base
    Collapse SRL Best Practices Series, Part 4:  Laboratory Safety in a Flow Cytometry SRL

    “Laboratory Safety in a Flow Cytometry Shared Resource Lab”

    This is the fourth of seven webinars in our SRL Best Practices Series

    Presented by:



    Joanne Lannigan, M.S.       
    Director, Flow Cytometry Core Facility
    University of Virginia School of Medicine
    Charlottesville, VA
    United States

     



    Dr. Desiree Kunkel
    Head of BCRT Flow Cytometry Lab
    Berlin-Brandenburg Center for
    Regenerative Therapies
    Germany

     

    About the Presenters

    Joanne Lannigan, M.S.

    Joanne Lannigan has been actively involved in Flow Cytometry for over 30 years, where she has worked in clinical, industry and academic flow cytometry laboratories.  She is currently the Director of the Flow Cytometry Core Facility in the School of Medicine at the University of Virginia.  She has been an active member of ISAC since 1996 and served as an ISAC Council Member from 2012-2016, Chair of the Shared Resource Laboratory Task Force from 2014-2016, a member of the Shared Resource Laboratory Services Oversight Committee from 2014-2016 and Vice Chair of the Certification Advisory Committee from 2014-2016. She currently is the Chair of the SRL Content Task Force of the Education Committee as well as a member of the Cytometry Board of Certification Committee through ASCP.  Ms. Lannigan’s current interests involve advancing technologies in cytometry, small particle analysis by flow cytometry, instrument evaluation and quality assurance, flow cytometry education and core management.

    Dr. Desiree Kunkel

    Dr. Kunkel has a Ph.D. in Immunology and did research for six years in the field of HIV/SIV-Immunology.  Flow Cytometry has been the major technology for her research and she took the opportunity to use her experience to become a full time flow cytometrist.  Since 2009, she has been the head of the BCRT Flow Cytometry Lab.  With her help, this lab was established from scratch and has since grown to a busy shared resource lab located at the Charite-Campus Virchow Klinikum in Berlin.  Her responsibilities include the development of quality assurance procedures and user training to ensure good data quality and reproducibility of results.  Desiree was part of the ISAC SRL Task Force and is a member of the Advisory Board of the German Society for Cytometry.

    Webinar Summary

    The importance of adequate safety practices within an SRL is critical to the overall function of such facilities. Working in a “shared” environment, in which a large variety of biological samples are introduced, requires a reasonable set of “best practices” to ensure the safety of everyone who accesses the shared facility. The best practices presented here represent what should be minimally present to provide a safe working environment.

    Learning Objectives

    Upon completion of this webinar, participants should be able to:

    • Describe the minimal practices for insuring a safe laboratory environment in a shared resource core lab
    • Assess the potential biosafety risks in an SRL or core lab
    • Develop a plan to minimize the exposure of staff and users to potential hazards
    • Consider the factors of the lab physical environment which are consistent with a safe work place
    • Recognize the importance of administrative factors in the assurance of a safe work environment


    Who Should Attend

    Directors, managers and staff of shared resource or core facilities
     

    Formats Available: Streaming, Live Webcast, Live Webcast + Streaming
    Original Seminar Date: May 30, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information SRL Best Practices Series, Part 4:  Laboratory Safety in a Flow Cytometry SRL
    Collapse SRL Best Practices Series, Part 3:  Training and Education

    “Training and Education”

    This is the third of seven in our SRL Best Practices Live Webinar Series

    Presented by:

     

     

    Michele Black
    Director, Cell Analysis Facility
    University of Washington

     

     

    James Marvin
    Director, Flow Cytometry Core Facility
    University of Utah Health Sciences Center

    About the Presenters:

    Michelle Black

    Michele Black has been working in the field of cytometry for 20 years. She was at Fred Hutchinson Cancer Research Center working in the Flow Lab from 1996 to 2013 as the Senior Flow Cytometry specialist. Michele was recruited by the Department of Immunology as the Technical Director of the Cell Analysis Facility at the University of Washington where she has held the position for the past 14 years. She has been an active member of ISAC since 1996 and has served on both the Shared Resource Laboratory Task Force and the SRL Services Committee. She is committed to providing quality flow cytometry services and education to researchers while increasing access to advanced technologies. She supports research activities while maintaining a fiscally sound core that consistently provides affordable, high quality service.

    James Marvin

    James Marvin has spent virtually his entire career within a Flow Cytometry shared resource setting.  In 2001, James joined the bustling flow lab at the University of Chicago under the tutelage of Julie Auger and Ryan Duggan. This was the era in which cell sorters were a bit more “hands on” and a firm understanding of the underlying hardware was essential on a daily basis. Next, James moved on to Northwestern University to “pick the brain” of Dr. Charles Goolsby.  In addition to the routine aspects involved in a flow core, a transition to more clinical assays was pursued during this time. Through a strong collaboration with industry as well as academic partners, significant strides were made in optimizing assays related to signal transduction analysis in normal as well as diseased bone marrow. Finally, in 2011, James uprooted from the Midwest in search of the mountains and desert of Utah where he has been the Director of the Flow Lab at the University of Utah in Salt Lake City. One of the many unifying themes through each of these distinct labs was the emphasis that education and training played for both facility staff and clients.

    Webinar Summary

    Education and training are essential parts of a SRL and cover a wide range of topics.  As cytometry SRLs are likely to be centered on technology and instrumentation, education within the lab should strongly focus on both the theoretical workings of the instrumentation as well as the specific details of running and acquiring samples for both staff and the end user. This Training and Education webinar aims to provide specialized guidance for best practices at SRL facilities across the globe and is the third of seven in our SRL Best Practices series. This series is not intended to specifically define how to implement these recommendations, but rather to help establish a set of goals for a SRL to achieve in order to reach its desired level of excellence.

    Learning Objectives

    A well balanced and thorough training and educational curricula within an SRL will touch on a very broad range of topics. The presenters will outline what should be addressed when developing an education program, such as:

    • Instrumentation
    • Experiment Design
    • Reagents
    • Lab Safety
    • Facility Policies
    • Data Analysis

    Creating an education and training program within a SRL will promote a consistent approach to introducing new staff and end users to a lab, and ensure that everyone has the appropriate knowledge to successfully utilize the instrumentation in the lab.

    Who Should Attend

    The intended audience for the discussion is staff of all levels associated with a flow cytometry core facility.

     

    Formats Available: Recording, Live Webinar, Live Webinar + Recording
    Original Seminar Date: April 12, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information SRL Best Practices Series, Part 3:  Training and Education
    Collapse SRL Best Practices Series, Part 2:  Standard Operating Procedures (SOPs)

    “SOPs - Why You Need Them”

    This is the second of seven webinars in our SRL Best Practices Series

    Presented by:


    Carina Torres
    Manager, Flow Cytometry Core
    Eli Lilly

     


    Benjamin J. Daniel

    Director, Flow Cytometry Shared Resource
    UT Health, San Antonio

     

    About the Presenters:

    Carina Torres

    Carina Torres has spent the past eighteen years as a shared resource professional in both academic and industry environments. Her current role as manager of the flow cytometry core at Eli Lilly in San Diego involves developing, implementing, and optimizing cytometry technologies to assist in antibody discovery and engineering platforms. She is co-founder of the San Diego Society for Cytometry and serves as a member of the Certification Advisory Committee and Shared Resources Lab Task Force for the International Society for the Advancement of Cytometry (ISAC).

    Benjamin J. Daniel

    Dr. Benjamin Daniel has been the Director of the Flow Cytometry Shared Resource at the UT Health, San Antonio for the past seven years. Prior to this, he studied how aging affects the immune responses to cancer.  He has been on the program committee for the past several CYTO conferences, along with the SRL Taskforce. He also currently serves as the VP for FlowTex, a Texas-based flow cytometry user group.

    Webinar Summary

    As the second of seven in the SRL Best Practices series, this live webinar will give the audience an overview of the importance of standard operating procedures (SOPs) and why they are important for all day-to-day activities. Several examples of SOPs will be discussed.

    Learning Objectives

    By the end of this webinar, viewers should understand the importance of SOPs, which ones are most important, and have general guidelines on how to author them.


    Who Should Attend

    SRL leaders and staff who want to learn more about the importance of SOPs.
     

    Formats Available: Streaming, Live Webcast
    Original Seminar Date: March 28, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information SRL Best Practices Series, Part 2:  Standard Operating Procedures (SOPs)
    Collapse SRL Best Practices Series, Part 1:  Managing Data in a Flow Cytometry Core

    “Managing Data in a Flow Cytometry Core”

    This is the first of seven webinars in our SRL Best Practices Series

    Presented by:



    Monica DeLay, MS                        
    Manager of flow Cytometry Lab Operations            
    Cincinnati Children’s Hospital

     



    Dr. Rachael Walker
    Head of Flow Cytometry
    Barbram Institute

    About the Presenters:

    Monica DeLay, MS

    Monica DeLay received her master’s degree in biology and afterwards, she performed research for over a decade in the area of autoimmune diseases focusing on the structure and function of human MHC and its role in modulating disease.  In 2008, she began managing the Research Flow Cytometry Core at Cincinnati Children’s Hospital in Cincinnati, Ohio.  Over the last nine years, she has helped facilitate the growth of the core in instrumentation, infrastructure, staff and clientele.  She is co-founder and president of the Ohio River Valley Cytometry Association, a local organization connecting advancements in cytometry technology to local scientists.  She is the current chair of the ISAC SRL Services Committee and co-chair of the SRL Emerging Leaders Committee.   She is a member and past co-chair of the ABRF Flow Cytometry Research Group whose mission is to understand alterations in cell function and develop best practices associated with cell sorting.  She is a member of the organizing committee for the Core Managers workshop for the Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA) and serves as a council member for ISAC.

    Dr. Rachael Walker

    Rachael Walker is the Head of Flow Cytometry at Babraham Institute, Cambridge, UK.  Rachael has over 11 years of experience with managing flow cytometry core facilities within Cambridge and has been in her current position since 2012.  She has a PhD in Tissue Engineering from the University of Liverpool, where she first learned to use a sorter and then embarked on a career in Flow Cytometry.   In 2012, Rachael was awarded an ISAC Scholarship and transferred to the new SRL Emerging Leaders program in 2014.  She is heavily involved in the flow cytometry community at a local level being Chair of the Mid-Anglia Cytometry Club; on a national level where she is Secretary of flowcytometryUK and is one of the main organizers of the Pre-CYTO ISAC Live Education Delivery Task Force.

    Webinar Summary

    This is the first of seven live webinars to focus on the SRL Best Practices series.  This webinar will give an overview of the important aspects in managing data in a Shared Resource Lab and provide examples of common practices used among some of the best SRLs from around the world.

    Learning Objectives

    At the completion of this webinar, attendees will understand key aspects when managing data in a flow cytometry core facility including standards for data collection and documentation, quality assurance, data preservation and computer maintenance.

    Who Should Attend

    SRL Staff, Managers or Directors wanting to learn about or improve on data management methods.

    CYTO U Webinar Recordings

    A recording of this webinar will be posted online at CYTO U within 24-48 hours after the live event for free viewing by all.  Recordings of previously presented live webinars are also available. 

    CYTO University

    CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, and interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.


     

    Formats Available: Streaming, Live Webinar, Webinar + Archive
    Original Seminar Date: March 16, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information SRL Best Practices Series, Part 1:  Managing Data in a Flow Cytometry Core
    Collapse Beyond the Bivariate: Towards a Flow Cytometry Computational Pipeline by Wade Rogers

    Wade Rogers

    Wade Rogers has been interested in pattern discovery for about 20 years.  It began with 'TupleWare', an algorithm for discovering patterns in gene and protein sequence data, first at DuPont and later at Bristol-Myers Squibb.  Later, he and colleagues expanded to include patterns in arbitrary categorical data.  For the past 10 years he has focused almost exclusively on the challenges of analyzing flow cytometry data at the University of Pennsylvania.

    Webinar Summary:

    Single cell analyses are becoming major players in life science markets.  However, the increasing dimensionality, complexity and volume of data produced by flow cytometry pose a serious challenge that must be overcome in order to realize the full clinical potential of the method. High dimensional cell-based measurements, coupled with sophisticated computational analysis (collectively termed “cytomics”) may yield phenotypic or functional patterns that can provide informative biomarkers for discovery and clinical use.  We envision an industrial-scale computational framework that will support the development and deployment of a data analysis pipeline that (a) is robust, hardened, and fully automated, eliminating analysis subjectivity and facilitating regulatory filing, (b), can be deployed in a centralized application-as-service business model for prospective, on-demand analysis of laboratory data and (c) enables retrospective datamining of cytomic data for analysis and discovery of new biomarkers.

    Formats Available: Streaming, Webinar + Archive
    Original Seminar Date: February 10, 2016
    On-Demand Release Date: Available Now

    Approved Credit:
  • ISAC: 1 hour ICCE
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Beyond the Bivariate: Towards a Flow Cytometry Computational Pipeline by Wade Rogers
    Collapse Bridging Flow Cytometry with New Technologies by Monica DeLay and Sherry Thornton. Moderated by Bridget Mclaughlin

    Monica DeLay

    Monica DeLay, MS

    After receiving her master’s degree in biology, she performed research for over a decade in the field of autoimmune diseases focusing on the structure and function of human MHC and its role in modulating disease.  Throughout that time she used flow cytometry for her research and in 2008 began managing the Research Flow Cytometry Core at Cincinnati Children’s Hospital in Cincinnati, Ohio.  Over the last seven years she has helped facilitate the growth of the core in instrumentation, infrastructure, staff and clientele.  She is co-founder and president of the Ohio River Valley Cytometry Association, a local organization connecting advancements in cytometry technology to local scientists.  She is co-chair of the ABRF Flow Cytometry Research Group whose mission is to understand alterations in cell function and develop best practices associated with cell sorting.  She is a member of the organizing committee for the Core Managers workshop for the Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA) and serves as a council member for the International Society for Advancement of Cytometry (ISAC).

    Sherry Thornton

    Sherry Thornton, Ph.D.

    Dr. Thornton received her Ph.D. in Developmental Biology from the University of Cincinnati College of Medicine. Her postdoctoral fellowship involved the use of flow cytometry in antigen-specific responses in the laboratory of Dr. Raphael Hirsch. She then joined the faculty in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center (CCHMC) and became flow core director for the Rheumatology Center Grant (NIH P30 AR47363). She has been extensively involved with the correlation of gene expression profiles with cell types (determined by flow cytometry analysis) of PBMC from large cohorts of Juvenile Idiopathic Arthritis patients (>500) and control specimens. Her research interests also include examination of cell types involved in the pathogenesis of arthritis. In June of 2008, Dr. Thornton led the consolidation of flow cytometry at CCHMC, and she became Director of the Research Flow Cytometry Core that houses all flow cytometry instrumentation involved in basic research at CCHMC. Dr. Thornton is active locally, nationally and internationally in the flow cytometry community. She is an active member of the International Society for Advancement of Cytometry, a founding member of the Ohio River Valley Cytometry Association, steering committee member of the Great Lakes International Imaging and Flow Cytometry Association and a member of the Career Development Committee of the Association of Biomolecular Resource Facilities.

    Webinar Summary:

    Novel applications, such as single-cell RNASeq, induced pluripotent stem cell (iPSC) generation and CRISPR-mediated genome editing, have revolutionized the way that cells can be analyzed and manipulated to investigate developmental pathways and disease processes.  These technologies can also benefit from using flow cytometry as an upstream or downstream process and, when combined, may require the involvement of several Shared Resource Labs (SRLs) from sample collection to data analysis. This webinar will highlight research projects that have utilized the above applications in combination with flow cytometry and/or cell sorting, discuss critical parameters that should be considered for successful experimental outcomes and provide strategies for coordination among core facility staff and investigators.  This discussion is aimed to provide ideas on the integration of new technologies from other cores into investigator-driven projects that utilize their SRL.

    -----------

    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit.  ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

    Formats Available: Streaming, Webinar + Archive
    Original Seminar Date: December 16, 2015
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Bridging Flow Cytometry with New Technologies by Monica DeLay and Sherry Thornton. Moderated by Bridget Mclaughlin
    Collapse Flow Cytometric Analysis of Endothelial Colony Forming Cells and Hematopoietic Progenitor Cells in Lung Vascular Disease by Kewal Asosingh and Imaging Flow Cytometry in the Study of Immune Cell Functions by Andrew Filby

    Kewal Asosingh

    Flow Cytometric Analysis of Endothelial Colony Forming Cells and Hematopoietic Progenitor Cells in Lung Vascular Disease presented by Kewal Asosingh (ISAC Scholar)

    Dr. Asosingh is a Staff Scientist, Assistant Professor of Molecular Medicine and Scientific Director for Flow Cytometry at Cleveland Clinic’s Lerner Research Institute. In 2011, he was chosen by the International Society for Advancement of Cytometry (ISAC) as an ISAC Scholar, recognition of emerging scientific research leaders in the profession. He has provided Stem Cell Cytometry training seminars at ISAC meetings, chaired oral presentation sessions on personalized medicine, and has been serving as abstract reviewer, member of the program committee for the annual ISAC meetings (“CYTO”) and reviewer for Cytometry Part A. Dr. Asosingh is also co-leader of the CYTO University (CYTO U) eLearning Delivery Task Force. Dr. Asosingh teaches flow cytometry to undergraduate and graduate students at the Cleveland Clinic and at Lakeland Community College. As Scientific Director of the Flow Cytometry Core, he serves as primary contact with investigators to provide advice on experimental design and data analysis/interpretation and assures that data generated by the Flow Core is of the highest quality.

    Webinar Summary:

    Dr. Asosingh’s lab studies bone marrow stem cells in pulmonary arterial hypertension (PAH) and pathological angiogenesis in asthma. The group has established mouse models for both diseases, and close collaboration with clinicians facilitates rapid transition of the basic research findings to clinical studies. In the past decade it has become evident that bone marrow hematopoietic stem cells exert functions beyond hematopoiesis. During development, endothelial cells and hematopoietic stem cells originate from a common bipotent mesodermal stem cell called the hemangioblast. In post-natal life, this connection is maintained via paracrine interactions between the endothelium and circulating hematopoietic progenitor cells. Their critical role in vascular homeostasis and repair of endothelial injury has opened new therapeutic perspectives for vascular degenerative diseases. The flipside of the coin is that abnormalities in hematopoiesis may underlie pathological angiogenesis, and this is exactly what his group is focused on.  Recent findings from the group show that hematopoietic stem cells isolated from the bone marrow of PAH patients are able to transfer the disease in humanized NOD SCID mice. PAH is a devastating disease characterized by endothelial cell injury, in situ thrombi and right ventricular hypertrophy. Animals engrafted with hematopoietic stem cells from PAH patients, but not from healthy control participants, had increased mobilization of progenitor cells in the peripheral blood, just like in patients, and strikingly developed many features of PAH.  Current focus of the team is to further reveal the mechanisms by which hematopoietic stem cells cause pulmonary vascular disease. This webinar will provide an overview of the different types of “endothelial progenitor cells” with special emphasis on endothelial colony forming cells and pro-angiogenic hematopoietic progenitor cells and their roles in PAH.

    Andrew Filby

    Imaging Flow Cytometry in the Study of Immune Cell Functions presented by Andrew Filby (SRL Emerging Leader)

    Dr Filby is currently head of the Flow Cytometry Core Facility at Newcastle University.  He leads a dedicated team of flow cytometry specialists with the sole aim of providing a comprehensive, cutting edge cytometry resource to the wider research community at Newcastle University and beyond.  A significant part of his focus is the development of novel cytometry-based techniques that have underpinned several high profile publications including in Science, Cell and the Cytometry Part A paper of the year accolade (2011).  He specialises in Imaging Flow Cytometry and the use of fluorescence dyes to track cell proliferation.  Prior to and post selection as an ISAC Shared Resource Laboratory Emerging Leader (SRLEL), Dr Filby has made several contributions to the Cyto conference programmes including interactive workshops and scientific talks.  He continues to contribute to the efforts of ISAC including serving on the Image Cytometry and E-learning task forces.  In addition to his work within ISAC, he is also heavily involved in supporting cytometry research and education in South America including initiatives for remote support and training.

    Webinar Summary:

    In this short webinar Dr Filby will give a brief overview of his work within the field of Imaging Flow Cytometry and what impact this has had on a number of key publications in the field of immunology and cell cycle biology.  These include the study of asymmetric cell division in the immune system, the measurement of organelle-specific calcium mobilisation in activated T cells and the analysis of the cell cycle in fission yeast.  In each case, the ability to capture and analyse literally thousands of multispectral, spatially registered images was essential to questions each method was designed to address.  Finally, Dr Filby will also discuss what the SRL EL programme means to him and how he hopes it will benefit the wider cytometry community through various initiatives he is involved in.

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    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

    Formats Available: Streaming, Webinar + Archive
    Original Seminar Date: November 16, 2015
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE
  • ISAC: 1 hour ICCE

  • Topics & Pricing InformationTopics & Pricing Information Flow Cytometric Analysis of Endothelial Colony Forming Cells and Hematopoietic Progenitor Cells in Lung Vascular Disease by Kewal Asosingh and Imaging Flow Cytometry in the Study of Immune Cell Functions by Andrew Filby
    Collapse Optimizing SRL Performance: Boost your Cell Sorting Capacity by Rui Gardner

    Rui Gardner, Gulbenkian Institute, Portugal

    Rui Gardner’s scientific career began just before graduating in Biochemistry in 1997, as a trainee in mathematical biology. This set the ground for his doctoral work, a mathematical approach to understand the prooxidative mechanisms of superoxide dismutase, paradoxically known as one of the most powerful physiological antioxidant enzymes. Most of the work was carried out in the department of Microbiology and Immunology at the University of Michigan, but also at the University of Southern California and the Gulbenkian Institute of Science in Portugal. In 2004, Rui earned his PhD in Biomedical Sciences, followed by postdoctoral work on evaluating immune diversity estimation techniques, still at the Gulbenkian Institute, where in the end of 2006 he became SRL Manager of the Flow Cytometry facility, job he still holds. Since 2007, Rui has been actively involved with ISAC’s SRL Task Force, and in 2012 was elected for ISAC’s Council, currently chairing the ISAC SRL Oversight Committee responsible for implementing and improving ISAC’s SRL-related activities and programs. In the last years, Rui has focused his efforts in addressing cell sorting performance based on the almost 10-year long experience running a cell sorting facility, as well as promoting meetings and discussions within the SRL community to help improve facility operations and management.  

     

    Webinar Summary:

    In the first of a series of webinars addressing strategies to optimize operations and management in a Shared Resource Lab (SRL) we’ll focus on enhancing cell sorting capacity. Many sorting facilities struggle with the increased demand in usage. The typically limited installed capacity of droplet cell sorters, which is directly associated to the requirement of a dedicated operator, usually leads to a narrow range of solutions to increase this capacity. Strategies are most often reduced to buying a new instrument, implying an increase in the number of FTEs or most likely increasing dramatically the burden of the current FTE, inevitably decreasing quality or SRL performance. With the advent of the new and more automated cell sorters, different and more creative solutions to increase cell sorting capacity are being addressed, including self-service sorting with all the implications associated to it. We’ll discuss several of these strategies, and present additional approaches to optimize usage of the current instrumentation, independent of their level of automation. These may include small technical implementations to reduce instrument setup, instrument troubleshooting guides, booking strategies to optimize resource usage, staggering shifts or “on call” staff to assist sorts that cannot be scheduled within normal sorting hours. There is no general strategy that will fit all facilities as some of these approaches may even collide with institutional or country-specific policies. Nevertheless, our aim is to lay down some of the possible strategies, open a discussion towards addressing this issue in a systematic way, and hopefully inspire new creative solutions from peers.

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    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

    Formats Available: Streaming, Webinar + Archive
    Original Seminar Date: October 29, 2015
    On-Demand Release Date: Available Now

    Approved Credit:
  • ISAC: 1 hour ICCE
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Optimizing SRL Performance: Boost your Cell Sorting Capacity by Rui Gardner
    Collapse Bridging Flow Cytometry with New Technologies

    Research projects frequently encompass technologies from more than one Shared Resource Lab (SRL). Novel applications, such as single-cell RNASeq downstream of cell sorting, may require the involvement of several SRLs from sample collection to data analysis. Often little to no communication between the client and the core staff occurs with regards to the experimental goals or the upstream and downstream processes related to a service request. This tutorial will discuss applications for which multiple core technologies are needed to complete a research project and strategies for effective communication, cooperation and coordination between staff of different cores. Upon conclusion of the tutorial, attendees will have specific tools to aid in the integration of new technologies from other cores into investigator-driven projects that utilize their SRL.

     

    Course Details:

    Research projects and applications using cytometry techniques that require cross-core collaboration such as cell sorting to single cell RNASeq using the Fluidigm C1 Critical parameters for experimental success Strategies for interaction between cores to support the investigator.

    Formats Available: Streaming

    Approved Credit:
  • ASCP: 1.50 hours CMLE
  • ISAC: 1.50 hours ICCE

  • Topics & Pricing InformationTopics & Pricing Information Bridging Flow Cytometry with New Technologies
    Collapse Flow Cytometry Data Management-Drinking from the Firehose (without Getting Wet)

    Flow Cytometry is a high-content technology, and unique in the complexity, not to mention the sheer volume of data of data that it generates. Typical experiments in the “pre-digital” era averaged in the 1-10 Mbyte range, whereas experiments done on modern digital instruments average over 100 times as much, and some extend well above 1000-fold larger. Techniques that were once adequate to manage data were not scalable, now leaving researchers and core facilities in a quandary: how do they store, organize, transfer, back up, archive and analyze these very large volumes of data? In this workshop we will get into the way-back machine and travel back to the time when storing and distributing data on CDs seemed so much better than floppy disks or ZIP drives. We will then pose the problem of today’s data in the context of yesterday’s technology and finally illustrate with examples some solutions that current computing technology, together with innovative software, have to offer.

     

    After the tutorial, the participant will understand the full dimension of the challenge of data management, and will be conversant in the technologies and strategies that can be utilized to effectively “drink from the firehose”.

     

    Course Details or Outline:

    • Historical Perspectives: How Did We Get Here?
    • Cytometry Instrumentation and Capabilities
    • Evolution of Computing Technologies
    • Current State: What Do We Do Now?
    • Cytometry Instrumentation and Capabilities
    • Modern Computing Technologies
    • Examples of Successful Data Management Strategies
    • A Glimpse into the Future
    • Summary/Conclusions

     

    Formats Available: Streaming

    Approved Credit:
  • ASCP: 1.50 hours CMLE
  • ISAC: 1.50 hours ICCE

  • Topics & Pricing InformationTopics & Pricing Information Flow Cytometry Data Management-Drinking from the Firehose (without Getting Wet)
    Collapse  Business Continuity and Risk Mitigation for Shared Resource Core Laboratories by Sheenah Mische

    Sheenah Mische

    Sheenah Mische, PhD, is the Senior Director in the Office of Collaborative Science. Dr. Mische oversees the Cores, providing a centralized administrative structure, and promoting state-of-the-art, service-oriented resources for the research community. Under the leadership of Associate Dean for Collaborative Science David Levy, she determines priorities for investment in new technologies, Cores, and services at the NYU Langone Medical Center. Dr. Mische has broad experience in the operation and administration of multi-technology, multidisciplinary resource centers in support of institutional research missions, both in academia and in industry.  In her most recent positions, she was Director of Translational Sciences, and Associate Director for Talent Acquisition and Academic Relations at Boehringer Ingelheim Pharmaceuticals. She was also previously Director of the Protein / DNA Technology Center at Rockefeller University.

    In recent years, research institutions and biomedical centers have suffered significant losses to research due to severe weather and other disasters.  Unfortunately, severe weather events are becoming more commonplace and Superstorm Sandy has brought climate impacts and risk into the conversation in ways not even contemplated before this October 2012 event.   The buzz word “business continuity planning” is a strategic plan to prevent, if possible, and to minimize and manage the consequences of an event that interrupts critical business processes.  From a biomedical research enterprise perspective, the business of research increasingly relies on centralized resource centers/cores, making them a critical and highly vulnerable component due to the concentration of instrumentation and resources.  This session will discuss business continuity planning, and both strategic and tactical considerations for emergency planning and risk mitigation.

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    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit.  ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

    Formats Available: Streaming
    Original Seminar Date: May 27, 2015
    On-Demand Release Date: Available Now

    Approved Credit:
  • ISAC: 1 hour ICCE
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information  Business Continuity and Risk Mitigation for Shared Resource Core Laboratories by Sheenah Mische
    Collapse Growing a Successful & Fruitful Core: You Reap What You Sow

    Joanne Lannigan has been actively involved in Flow Cytometry for over 30 years. She served as the Associate Technical Director of the Clinical Immunology and Flow Cytometry Laboratories at University Hospital at the State University of New York at Stony Brook from 1986-1999 where she supervised the clinical immunology and flow cytometry patient testing services. In 2002 she joined the Faculty of the Department of Microbiology, Immunology and Cancer Biology in the School of Medicine at the University of Virginia as a Senior Research Scientist and Director of the Flow Cytometry Core Facility. She has been an active member of ISAC since 1996 and is currently an ISAC Council member, Chair of the Shared Resource Laboratory Task Force, member of the Shared Resource Laboratory Services Oversight Committee, and Vice Chair of the Certification Advisory Committee. Ms. Lannigan's current interests involve advancing technologies in cytometry, instrument evaluation and quality assurance, flow cytometry education and core management.

    The keys to a successful research core facility are a shared responsibility between the institutional research administration and the individual core directors. Both play important roles in providing the necessary resources and strategies to insure the research missions of the cores are efficiently and effectively met. This requires the alignment of goals and coordination of efforts in business planning, forecasting, and marketing. The presenter will share some personal experiences and approaches to the every day challenges of growing a successful core. We will address such challenges as:

    • Developing the right core environment
    • The Customer-Core relationship
    • Expense recovery vs. affordable rates
    • Growing the user base
    • Establishing an efficient (lean) operation
    • Recruiting/retaining quality staff
    • Ensuring quality data
    • Planning for the future

    Approaching these challenges from a day-to-day perspective is an important part of effectively integrating the larger goals of the institution; no one knows your strengths and weaknesses better than you.

    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit.  ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

    Formats Available: Streaming
    Original Seminar Date: February 24, 2015
    On-Demand Release Date: Available Now

    Approved Credit:
  • ISAC: 1 hour ICCE
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Growing a Successful & Fruitful Core: You Reap What You Sow
    Collapse Forensic Flow Cytometry

    Jennifer Wilshire will be presenting the webinar "Forensic Flow Cytometry." Jennifer is the Assistant Manager of the Flow Cytometry Core Facility at Memorial Sloan-Kettering Cancer Center in New York, NY.

    Do you sometimes look at flow cytometry results and wonder if something went wrong, but can't put your finger on it? In this webinar we will take a "forensic" approach to study the most common issues that give bad flow cytometry data.  We will use case studies and real examples to illustrate the causes of bad data and how you can prevent it. Many topics will be covered including staining issues, compensation, controls, and sorting. The focus of this webinar will be on understanding the potential pitfalls in flow cytometry experiments and learning practical methods to prevent these issues. After participating in this webinar, attendees will have practical suggestions of ways to ensure flow cytometry experiments will yield good data and purely sorted cells.

    Formats Available: Streaming
    Original Seminar Date: August 05, 2014
    On-Demand Release Date: Available Now

    Approved Credit:
  • ISAC: 1 hour ICCE
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Forensic Flow Cytometry
    Collapse Finances 101: Budgets, Service Fees & Equipment Service Costs (2014 Scientific Tutorial)
    Every good core facility director recognizes that her or his facility operates as a small business. However, most core directors are trained as scientists, not business specialists. During this tutorial we will provide a basic interactive hands on tutorial reviewing the financial management of a core facility.  This will provide participants with the information they need manage equipment maintenance expenses, calculate service fees and understand their annual operating budget. It will also prepare them for successful conversations with their financial manager.
     
    After participating in this tutorial, the student will leave with various tools including an equipment maintenance workbook to make an informed decision regarding service contract versus self-insurance. They will also have a workbook to calculate service fees and create an annual operating budget.
    Formats Available: Streaming

    Approved Credit:
  • ISAC: 1.50 hours ICCE
  • ASCP: 1.50 hours CMLE

  • Topics & Pricing InformationTopics & Pricing Information Finances 101: Budgets, Service Fees & Equipment Service Costs (2014 Scientific Tutorial)
    Collapse Enhancing the Shared Resource Laboratory through the Use of Social Media

    Ryan Duggan

    Ryan Duggan will be presenting the webinar "Enhancing the Shared Resource Laboratory through the Use of Social Media." Ryan serves as the Technical Director of the Flow Cytometry Core Facility at the University of Chicago (UCFlow).

    The past decade has seen a dramatic emergence of new media forms (such as social media) and web-(or"cloud") based tools that have had a significant impact on the ways in which individuals and organisations interact and conduct business. Like other areas of science and industry, shared resource laboratories (SRLs) have much to gain from leveraging these technologies. This webinar will share some of my experiences in utilising these tools. New media will be discussed in the context of enhancing SRL visibility and status and interacting with colleagues and customers. These tools will give your facility renewed confidence in providing high quality services in an efficient manner while expanding your user base within and beyond your own Institution.

    Formats Available: Streaming
    Original Seminar Date: April 16, 2014
    On-Demand Release Date: Available Now

    Approved Credit:
  • ISAC: 1 hour ICCE
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Enhancing the Shared Resource Laboratory through the Use of Social Media
    Collapse Growing a Cytometry Core Facility: Adding Value with Hardware and Education: Innovation (2013 Scientific Tutorials PreCongress Course)
    Core facilities are now common in all work settings. Flow cytometry is a well-established technique but the core faces particular challenges in the face of expanding technology. In particular, cores need to bring added value to their users and institutional setting. But how can core facility staff keep up with the latest developments, how can they receive appropriate continuing education and how can this be passed on to users of a facility? We will discuss evaluation of technology and strategies for importing this into a core and also how education on site, at relevant meetings and by remote learning can benefit the facility. At the end of the tutorial the delegate will be aware of the approaches that can be taken to bring added value to the core, its staff and its users.


    All CYTO U courses qualify for ICCE continuing education credit.
    Formats Available: Streaming
    Original Seminar Date: May 18, 2013
    On-Demand Release Date: Available Now

    Approved Credit:
  • ISAC: 1.50 hours ICCE
  • ASCP: 1.50 hours CMLE

  • Topics & Pricing InformationTopics & Pricing Information Growing a Cytometry Core Facility: Adding Value with Hardware and Education: Innovation (2013 Scientific Tutorials PreCongress Course)